|Queen of Blood, Part 1|
|Season 8, Episode 1|
|I Will Always Protect You, No Matter What||Queen of Blood, Part 2|
PART 1: REGIME CHANGE- Mary Seward returns and makes short work of her opposition at L-Corp, causing Lena Luthor to retaliate. However, she could be playing directly into Mary's hands. Meanwhile, Lucy Lane considers becoming Superwoman once more and Diana, Princess of Themyscira moves to National City.
- Melissa Benoist as Kara Danvers/Supergirl & Red Daughter
- Chyler Leigh as Alex Danvers
- Katie McGrath as Lena Luthor
- Odette Annable as Samantha Arias
- Olivia Wilde as Susannah Russo
- Jenna Dewan as Lucy Lane
- Adelaide Kane as Ava Blackwood
- with Minka Kelly as Dawn Granger
- and Emmy Rossum as Zaora Dru-Zod
- Brenda Strong as Lillian Luthor
- Troian Bellisario as Mary Seward
- Dichen Lachman as Talia al Ghul (Earth-27)
- Marie Avgeropoulos as Diana, Princess of Themyscira
- Teressa Liane as Mary Louise Fairchild
- Scarlett Byrne as Nora Fairchild
- Brit Morgan as Livewire
- Wallis Day as Chief Magistrate Nyssa-Vex
- Tamzin Merchant as Lyra Strayd
- and Oded Fehr as Ra's al Ghul
- Antagonist: Mary Seward
- This episode is Lena-centric.
- First appearance of Ava Blackwood.
- The first episode to not feature Clark, Karen, and J'onn as series regulars.
- It is now February of 2024. Seven months have passed since Kara and Lena's wedding.